Dissemination of NDM-1 in pseudomonas aeruginosa and klebsiellapneumoniae isolated from pus samples in tertiary care hospitals of Quetta, Pakistan

Authors

  • Mohammad Din Department of Pathology, Bolan Medical College/Complex Hospital, Quetta, Pakistan
  • Khan Muhammad Babar Department of Surgery, Bolan Medical College/Complex Hospital, Quetta, Pakistan
  • Dawood Shah Department of Cardiac Surgery, Bolan Medical College/Complex Hospital, Quetta, Pakistan
  • Ghulam Sarwar Department of Pathology, Bolan Medical College/Complex Hospital, Quetta, Pakistan
  • Saleem Khan Department of Neuro Surgery Bolan Medical College/Complex Hospital, Quetta, Pakistan
  • Hummaira Sadaf Department of Biotechnology, Sardar Bahadur Khan Women University, Quetta, Pakistan
  • Shahjahan Shabbir Ahmed Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan
  • Faiza Liaqat Ali CASVAB University of Balochistan Quetta, Pakistan
  • Muhammad Yasir School of Optometry and Vision Science, University of New South Wales, Australia
  • Nazeer Ahmed Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan

DOI:

https://doi.org/10.47391/JPMA.259

Abstract

Abstract

Objective: To highlight the prevalence and epidemiology of New Delhi metallo-ß-lactamase-1.producers in pus samples.

Methods: The cross-sectional study was conducted from April to August 2018at the Biotechnology Laboratory, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, Hi-tech Laboratory, Centre for Advance Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, and Microbiology Laboratory, Bolan Medical Complex Hospital, Quetta, Pakistan. Biochemical and molecular approaches were used for the identification of the isolates and Modified Hodge Test for the phenotypic detection of class-A carbapenemase activity. Minimum Inhibitory Concentration was performed using E-test and broth microdilution method. Molecular basis of carbapenemase activity was ascertained by the recognition of blaNDM-1 gene in the isolates.

Results: Of the 300 pus samples taken from surgical/burn units,6(2%) blaNDM-1 harbouring isolates were found; 3(50%) each being Klebsiellapneumoniae andPseudomonas aeruginosa. Klebsiella. pneumoniaeisolates were extensivelydrug-resistant. The Pseudomonas aeruginosaisolates displayed resistance against 21 antibiotics of tetracyclines, quinolones, ?-lactams, aminoglycosides, monobactams, sulphonamides, macrolides, cephalosporins, phosphonic acid and polypeptide groups, suggesting pan-drug resistance.

Conclusion: The resistance pattern of the bacterial isolates poses a significant clinical threat in the region.

Key Words: blaNDM-1, Carbapenemase, XDR, PDR, Quetta.

Published

2020-10-27

Issue

Section

Research Article