MicroRNA miR-425 inhibits proliferation and migration of colorectal cancer HCC116 cells

Authors

  • Huifang Zhou Department of Clinical Laboratory, The First People's Hospital in Kashi, Affiliated Kashi Hospital of Sun Yat-Sen University, Kashi, China
  • Wen Liu Department of Clinical Laboratory, The First People's Hospital in Kashi, Affiliated Kashi Hospital of Sun Yat-Sen University, Kashi, China
  • Haiyun Chen Department of Clinical Laboratory, The First People's Hospital in Kashi, Affiliated Kashi Hospital of Sun Yat-Sen University, Kashi, China
  • Jiajia Ni Research and Development Center, Guangdong Meilikang Bio-Science Ltd., Dongguan, China
  • Zhi Zhang Department of Clinical Laboratory, The Second People;s Hospital of Guangdong Province, Guangzhou, China
  • Aimin Xu Department of Clinical Laboratory, The First People's Hospital in Kashi, Affiliated Kashi Hospital of Sun Yat-Sen University, Kashi, China

DOI:

https://doi.org/10.47391/JPMA.02-218

Abstract

Abstract

Objective: To verify the role of miR-425 in colorectal carcinogenesis.

Methods: We conducted sphere formation assay, wound-healing assay, transwell assay, MTT cell proliferation assay, flow cytometric analysis, reverse transcription qPCR, and Western blotting to analyse proliferation and invasion of HCT116 cells transfected with miR-425 mimics, miR-425 inhibitor, miR-425 mimic negative control, and miR-425 inhibitor negative control, respectively. The experimental protocol was approved by the Medical Ethics Committee of the First People’s Hospital in Kashi (approval number: 2018 Kuaishen No. (100)). All experiments were conducted during December 2016 to July 2017 at Xinjiang Dingju Medical Laboratory, China.

Results: Our results showed that miR-425 expression in HCT116 cells after transfection was up-regulated, which inhibited sphere formation. Overexpression of miR-425 inhibited proliferation of HTC116 cells and induced their apoptosis, and inhibited HCC116 cell migration and invasion.

Conclusion: Overexpression of miR-425 could inhibited sphere formation, the migration and invasion of HCT116 cells through inhibiting proliferation and promoting apoptosis.

Keywords: apoptosis; colonic carcinoma; microRNA; proliferation

Continuous....

Published

2021-07-06

Issue

Section

Research Article