X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression

Authors

  • Jin-xiao Liang Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China
  • Wei Gao School of Medicine, Zhejiang University City College, China
  • Wei-tian Wei Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China
  • Xun Yang Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China
  • Jin-shi Liu Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China

DOI:

https://doi.org/10.47391/JPMA.5312

Abstract

Objective: To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation.

Method: The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5.

Results: The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y.

Conclusion: There was a significant role of glycosylation in radiation therapy for lung cancer.

Key Words: Fucosyltransferase 4, SP1, X-ray, Lung cancer, Cell proliferation.

Published

2023-03-04

How to Cite

Jin-xiao Liang, Gao, W., Wei, W.- tian, Yang, X., & Liu, J.- shi. (2023). X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression. Journal of the Pakistan Medical Association, 73(3), 494–499. https://doi.org/10.47391/JPMA.5312

Issue

Section

Research Article