@article{Maria Rukan_Humaira Jamil_Habib Ali Bokhari_Aamer Ali Khattak_Allah Nawaz Khan_Zahid Ullah_Sarfaraz Hussain_Muhammad Ali Syed_2020, title={Nasal carriage of highly resistant methicillin resistant Staphylococcus aureus (MRSA) strains by hospital staff in Hazara region of Pakistan}, url={https://ojs.jpma.org.pk/index.php/public_html/article/view/1751}, DOI={10.47391/JPMA.177}, abstractNote={<p><strong><u>Abstract</u></strong></p> <p><strong>Objective: </strong>To isolate and characterise multidrug resistant strains of <em>Staphylococcus aureus</em> from healthcare workers who are at potential risk of nosocomial infections.</p> <p><strong>Methods: </strong>The observational, cross-sectional study was conducted from November 2014 to April 2015 at different hospitals of Haripur and Abbottabad, Pakistan, and comprised ward and operation theatre staff. The isolates were identified on the basis of microbiological and biochemical tests and further confirmed by polymerase chain reaction. Disc diffusion method was used for antibiotic sensitivity testing, and panton valentine leukocidin and methicillin resistance mecA genes were detected using polymerase chain reaction.</p> <p><strong>Results: </strong>Of 208 isolates, 108(52%) were from the ward staff and 100(48%) were from the operation theatre staff. Overall, 167(80.3%) isolates were positive for <em>Staphylococcus aureus</em>, and 75(36%) were methicillin-resistant <em>Staphylococcus aureus</em><em>. </em>The number of antibiotic-resistant isolates was 75(45%) cefoxitin, 60(36%) ofloxacin, 152(91%) erythromycin, 52(31%) doxycycline, 127(76%) lincomycin, 53(32%) amoxicillin-clavulanate, 67(40%) ciprofloxacin, and 89(53%) ceftriaxone.</p> <p><strong>Conclusion: </strong>A high number of hospital staff, including those working in operation theatres, were found to be carrying methicillin-resistant <em>Staphylococcus aureus</em> and multidrug resistant strains in their nasal passage that may be a source of infection to patients.</p> <p><strong>Key Words: </strong>Methicillin resistance, Nosocomial infections, Vancomycin, MecA gene, Pvl gene. <strong>Continuous....</strong></p>}, journal={Journal of the Pakistan Medical Association}, author={Maria Rukan and Humaira Jamil and Habib Ali Bokhari and Aamer Ali Khattak and Allah Nawaz Khan and Zahid Ullah and Sarfaraz Hussain and Muhammad Ali Syed}, year={2020}, month={Oct.}, pages={1–12} }