TY - JOUR AU - Sidra Asad Ali, AU - Bushra Moiz , AU - Lumaan Sheikh, PY - 2021/12/29 Y2 - 2024/03/29 TI - Association of factor V Leiden G1691A and prothrombin gene G20210A mutations with adverse pregnancy outcomes JF - Journal of the Pakistan Medical Association JA - J Pak Med Assoc VL - 71 IS - 7 SE - Research Article DO - 10.47391/JPMA.1377 UR - https://ojs.jpma.org.pk/index.php/public_html/article/view/865 SP - 1780-1784 AB - <p><strong>Objective:</strong> To determine the association of Factor V Leiden / prothrombin gene mutation in Pakistani women with adverse pregnancy outcomes.</p><p><strong>Method:</strong> The prospective study was conducted at the Aga Khan University Hospital, Karachi, from January 1 to December 31, 2016, and comprised females ?40 years having history of two or more foetal losses with no apparent aetiology. Restriction fragment length polymorphism- Polymerase chain reaction was performed using MnlI and HindIII restriction enzymes for factor V Leiden G1691A and prothrombin gene mutation G20210A. Females with two or more consecutive normal pregnancies were enrolled as the control group. Data was analysed using SPSS 19.</p><p><strong>Results: </strong>Of the 172 participants with a mean age of 29.3±5.9 years (range: 19-38 years). 86(50%) each were healthy controls and those with recurrent pregnancy loss. There were 238 livebirths among the controls compared to 13 in the other group. Factor V Leiden G1691A was identified in 2(2.3%) women, and prothrombin gene mutation G20210A in 1(1.2%) woman in the patient group, while no mutation was identified in the control group.</p><p><strong>Conclusion:</strong> The prevalence of Factor V Leiden / prothrombin gene mutation in women with recurrent pregnancy loss was found to be very low.</p><p><strong>Continuous....</strong></p> ER -